Determining Minimum Inhibitory Concentrations in Liquid Cultures or on Solid Medium

Methods Mol Biol. 2021;2314:595-609. doi: 10.1007/978-1-0716-1460-0_26.


Antimicrobial susceptibility testing is the mainstay of tuberculosis drug development programs. In this chapter, we describe methods for determination of the minimum inhibitory concentration of compounds against Mycobacterium tuberculosis growing in liquid media as a function of carbon source, detergent, and environmental stress imposed by acidic pH as well as reactive nitrogen intermediates. Methods for determining the effect of bovine serum albumin in the growth medium on antimicrobial susceptibility are also described. Finally, we provide a method for antimicrobial susceptibility testing on agar medium.

Keywords: Acidified medium; Agar MIC; Carbon source; Detergent; Liquid MIC; Minimum inhibitory concentration (MIC); Nitrosative stress.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Agar / chemistry*
  • Antitubercular Agents / pharmacology*
  • Carbon / metabolism
  • Culture Media / pharmacology*
  • Humans
  • Hydrogen-Ion Concentration
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / growth & development*
  • Serum Albumin, Bovine / metabolism*
  • Stress, Physiological
  • Tuberculosis / drug therapy*
  • Tuberculosis / microbiology
  • Tuberculosis / pathology


  • Antitubercular Agents
  • Culture Media
  • Serum Albumin, Bovine
  • Carbon
  • Agar