Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Oct;47(10):3471-3479.
doi: 10.1111/jog.14927. Epub 2021 Jul 7.

Formyl peptide receptor-2 is upregulated in the blood and placenta of patients with gestational diabetes mellitus

Wenfeng Ye  1 , Linlin Chen  1 , Yanjun Yang  1 , Changfang Yao  1 , Lihua Zhu  1 , Qi Wang  2 , Jingting Jiang  2
Affiliations
Free PMC article

Formyl peptide receptor-2 is upregulated in the blood and placenta of patients with gestational diabetes mellitus

Wenfeng Ye et al. J Obstet Gynaecol Res. 2021 Oct.
Free PMC article

Abstract

Aim: To investigate the expression of formyl peptide receptor 2 (FPR2) in maternal blood, umbilical blood, and placenta of patients with gestational diabetes mellitus (GDM), and to analyze the changes of other pro-inflammatory cytokines in blood, including interleukin 33 (IL-33), IL-1β, tumor necrosis factor alpha (TNF-α), and C-reactive protein (CRP), so as to reveal the pathogenesis of GDM.

Methods: FPR2, IL-33, IL-1β, T TNF-α, and CRP in maternal blood and umbilical cord blood of 50 pregnant women with GDM and 30 normal pregnant women were analyzed by ELISA method to explore the correlation between inflammatory factors and blood glucose. The expression of FPR2 in placental tissues was analyzed by PCR and immunohistochemistry.

Results: The expression of FPR2 in maternal blood of gestational diabetes patients was significantly higher than that of normal pregnant women, and other inflammatory factors IL-33 and IL-1β in maternal blood were also significantly increased. The expression of FPR2 in umbilical cord blood of gestational diabetes was higher than that of normal pregnant women, but the difference was not significant. Other inflammatory factors IL-33, IL-1β, and CRP in umbilical cord blood were also significantly increased. The expression of FPR2mRNA and protein in placental tissues of gestational diabetes was significantly higher than that of normal pregnant women.

Conclusions: The level of FPR2, IL-33, and IL-1β in maternal blood was related to the pathogenesis of GDM and these inflammatory factors could be used as special candidate direction of marks for the prevention, clinical treatment and drug design of GDM, laying a new theoretical foundation for the treatment of GDM.

Keywords: FPR2; GDM; inflammatory cytokines; pathogenesis.

PubMed Disclaimer

Conflict of interest statement

All authors declare no conflict of interest for this work.

Figures

Figure 1
Figure 1
FPR2 expression with PCR is shown. Normal: placenta from healthy pregnant women. GDM: placenta from GDM women. p < 0.05
Figure 2
Figure 2
FPR2 and inflammatory mediators in the maternal peripheral blood with ELISA are shown. Results are presented as mean ± SD. Normal = 30, GDM = 50,*p < 0.05, **p < 0.01 (Student's t test)
Figure 3
Figure 3
FPR2 and inflammatory mediators in the umbilical blood with ELISA are shown. Results are presented as mean ± SD. Normal = 30, GDM = 5O, *p < 0.05, **p < 0.01 (Student's t test)
Figure 4
Figure 4
FPR2 immunohistochemical staining. (a) GDM; (b) normal pregnancy
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. HAPO Study Cooperative Research Group , Metzger BE, Lowe LP, et al. Hyperglycemia and adverse pregnancy outcomes. N Engl J Med. 2008;358:1991–2002. - PubMed
    1. American Diabetes Association . 2. Classification and diagnosis of diabetes. Diabetes Care. 2017;40:S11–24. - PubMed
    1. Leng J, Shao P, Zhang C, et al. Prevalence of gestational diabetes mellitus and its risk factors in Chinese pregnant women: a prospective population‐based study in Tianjin, China. PLoS One. 2015;10:e0121029. - PMC - PubMed
    1. Fetita LS, Sobngwi E, Serradas P, et al. Consequences of fetal exposure to maternal diabetes in offspring. J Clin Endocrinol Metab. 2006;91:3718–24. - PubMed
    1. Clapp JF. Effects of diet and exercise on insulin resistance during pregnancy. Metab Syndr Relat Disord. 2006;4:84–90. - PubMed