Macrophages are an abundant population in the tumor-infiltrating immune cells. The transcription factor NF-κB plays an important role in the response of tumor-associated macrophages (TAMs) to the tumor environmental cues. Detecting NF-κB activity in TAMs will help define the functional status of the TAMs. In this article, we describe several methods to detect NF-κB activity in TAM populations.
Keywords: Chromatin immunoprecipitation sequencing (ChIP-Seq); Immunofluorescence (IF); Immunohistochemistry (IHC); NF-κB; cancer; RNA sequencing; Tumor-associated macrophages (TAMs).