Screening of polymeric microplastic debris can help to assess the extent to which plastics contaminate the environment. We here developed an easy- and rapid-to-perform method for the screening of plastic polymers, based on a newly employed fluorophore, namely 1-pyrenebutyric acid N-hydroxysuccinimidyl ester (PBN). The PBN fluorophore was capable of staining diverse synthetic microplastic polymers within 5 min, including those displaying various particle sizes and shapes. The fluorescence intensities of the microplastics were considerably enhanced after the short-duration staining. The screening method was shown to be highly effective in the detection of polyethylene (PE), poly(ethylene terephthalate) (PET), polyvinyl chloride (PVC), polyamide-6 (PA-6), polypropylene (PP), polystyrene (PS), polycarbonate (PC), polyvinylidenechloride (PVDC), and polyurethane (PU), with a lowest analyzed particle size of 15 μm. Using our screening method, plastic contamination was investigated in commercially available bottled waters and environmental waters, specifically urban freshwaters. This study demonstrated high affinity levels of the newly proposed PBN fluorophore for a broad range of polymers and its ability to be used to discernibly identify polymer particles.
Keywords: Microbeads; Mineral water; Plastic contamination; Urban stream.
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