Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/ FNIP2 Axis in Chicken Adipogenesis

Lijin Guo; Xiaohuan Chao; Weiling Huang; Zhenhui Li; Kang Luan; Mao Ye; Siyu Zhang; Manqing Liu; Hongmei Li; Wen Luo; Qinghua Nie; Xiquan Zhang; Qingbin Luo

doi:10.3389/fcell.2021.653798

Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/ FNIP2 Axis in Chicken Adipogenesis

Front Cell Dev Biol. 2021 Jun 24:9:653798. doi: 10.3389/fcell.2021.653798. eCollection 2021.

Authors

Lijin Guo^{1

2}, Xiaohuan Chao^{1

2}, Weiling Huang³, Zhenhui Li^{1

2}, Kang Luan^{1

2}, Mao Ye^{1

2}, Siyu Zhang^{1

2}, Manqing Liu^{1

2}, Hongmei Li^{1

2}, Wen Luo^{1

2}, Qinghua Nie^{1

2}, Xiquan Zhang^{1

2}, Qingbin Luo^{1

2}

Affiliations

¹ Guangdong Laboratory, Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China.
² College of Animal Science, South China Agricultural University, Guangzhou, China.
³ School of Biological and Chemical Sciences, Queen Mary University of London, London, United Kingdom.

Abstract

Lipid biosynthesis is a complex process, which is regulated by multiple factors including lncRNA. However, the role of lncRNA in chicken abdominal fat accumulation is still unclear. In this research, we collected liver tissues from six high abdominal fat rate Sanhuang broilers and six low abdominal fat rate Sanhuang broilers to perform lncRNA sequencing and small RNA sequencing. A total of 2,265 lncRNAs, 245 miRNAs, and 5,315 mRNAs were differently expressed. Among of them, 1,136 differently expressed genes were enriched in the metabolic process. A total of 36 differently expressed genes, which were considered as differently expressed lncRNAs' targets, were enriched in the metabolic process. In addition, we also found out that eight differently expressed miRNAs could target 19 differently expressed genes. FNIP2 and PEX5L were shared in a cis-regulatory network and a differently expressed miRNA target relationship network. LncRNA-FNIP2/miR-24-3p/FNIP2 axis was considered as a potential candidate that may participate in lipid synthesis. Experimentally, the objective reality of lncRNA-FNIP2/miR-24-3p/FNIP2 axis was clarified and the regulation effect of lncRNA-FNIP2/miR-24-3p/FNIP2 axis on synthesis was validated. In brief, our study reveals a potential novel regulatory mechanism that lncRNA-FNIP2/miR-24-3p/FNIP2 axis was considered as being involved in lipid synthesis during chicken adipogenesis in liver.

Keywords: adipogenesis; lipid synthesis; lncRNA; mRNA; miRNA; whole transcriptome analysis.