In Silico Prediction for ncRNAs in Prokaryotes

Methods Mol Biol. 2021:2328:277-285. doi: 10.1007/978-1-0716-1534-8_18.


The identification and characterization of non-coding RNAs (ncRNAs) in prokaryotes is an important step in the study of the interaction of these molecules with mRNAs-or target proteins, in the post-transcriptional regulation process. Here, we describe one of the main in silico prediction methods in prokaryotes, using the TargetRNA2 tool to predict target mRNAs.

Keywords: Prediction in silico; Prokaryote; ncRNAs.

MeSH terms

  • Computational Biology / methods*
  • Computer Simulation
  • Databases, Genetic
  • Gene Expression Profiling / methods*
  • Genome, Microbial / genetics*
  • Molecular Sequence Annotation
  • Prokaryotic Cells / metabolism*
  • RNA, Untranslated / genetics
  • RNA, Untranslated / metabolism*
  • RNA-Seq
  • Software


  • RNA, Untranslated