Homeostatic functions of monocytes and interstitial lung macrophages are regulated via collagen domain-binding receptor LAIR1

Immunity. 2021 Jul 13;54(7):1511-1526.e8. doi: 10.1016/j.immuni.2021.06.012.


Myeloid cells encounter stromal cells and their matrix determinants on a continual basis during their residence in any given organ. Here, we examined the impact of the collagen receptor LAIR1 on myeloid cell homeostasis and function. LAIR1 was highly expressed in the myeloid lineage and enriched in non-classical monocytes. Proteomic definition of the LAIR1 interactome identified stromal factor Colec12 as a high-affinity LAIR1 ligand. Proteomic profiling of LAIR1 signaling triggered by Collagen1 and Colec12 highlighted pathways associated with survival, proliferation, and differentiation. Lair1-/- mice had reduced frequencies of Ly6C- monocytes, which were associated with altered proliferation and apoptosis of non-classical monocytes from bone marrow and altered heterogeneity of interstitial macrophages in lung. Myeloid-specific LAIR1 deficiency promoted metastatic growth in a melanoma model and LAIR1 expression associated with improved clinical outcomes in human metastatic melanoma. Thus, monocytes and macrophages rely on LAIR1 sensing of stromal determinants for fitness and function, with relevance in homeostasis and disease.

Keywords: COLEC12; LAIR1; cancer; collagen; lung; macrophage; monocyte; myeloid homeostasis; stroma.

MeSH terms

  • Animals
  • Apoptosis / physiology
  • Bone Marrow / metabolism
  • Bone Marrow / pathology
  • COS Cells
  • Cell Differentiation / physiology
  • Cell Line
  • Cell Line, Tumor
  • Cell Lineage / physiology
  • Cell Proliferation / physiology
  • Chlorocebus aethiops
  • Female
  • Homeostasis / physiology*
  • Humans
  • Lung / metabolism*
  • Lung / pathology
  • Macrophages, Alveolar / metabolism*
  • Macrophages, Alveolar / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Monocytes / metabolism*
  • Monocytes / pathology
  • Myeloid Cells / metabolism
  • Myeloid Cells / pathology
  • Neoplasm Metastasis / pathology
  • Proteomics / methods
  • Receptors, Immunologic / metabolism*
  • Signal Transduction / physiology


  • Receptors, Immunologic
  • leukocyte-associated immunoglobulin-like receptor 1