Molecular cloning, cDNA structure and deduced amino acid sequence for a type I regulatory subunit of cAMP-dependent protein kinase from human testis

M Sandberg; K Taskén; O Oyen; V Hansson; T Jahnsen

doi:10.1016/0006-291x(87)90499-2

Molecular cloning, cDNA structure and deduced amino acid sequence for a type I regulatory subunit of cAMP-dependent protein kinase from human testis

Biochem Biophys Res Commun. 1987 Dec 31;149(3):939-45. doi: 10.1016/0006-291x(87)90499-2.

Authors

M Sandberg¹, K Taskén, O Oyen, V Hansson, T Jahnsen

Affiliation

¹ Institute of Pathology, Rikshospitalet, Oslo, Norway.

PMID: 3426618
DOI: 10.1016/0006-291x(87)90499-2

Abstract

A 1.5 kilobase (kb) cDNA clone containing the entire coding region for a regulatory subunit of type I cAMP-dependent protein kinase (RI) was isolated from a human testis cDNA library. The cDNA clone encodes a protein of 381 amino acids that shows 98% and 97% homology to the bovine skeletal muscle RI and rat brain RI, respectively. Northern blot analysis demonstrates two major mRNA-species (1.5 and 3.0 kb) in human testis and one mRNA-species (3.0 kb) in human T-lymphocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cattle
DNA / genetics*
Humans
Male
Molecular Sequence Data
Protein Kinases / genetics*
RNA, Messenger / analysis
Rats
Sequence Homology, Nucleic Acid
Testis / enzymology*

Substances

RNA, Messenger
DNA
Protein Kinases

Associated data

GENBANK/M18468