Haploid and Doubled Haploid Plant Production in Brassica rapa L. subsp. pekinensis Via Microspore Culture

Adela Adamus; Marek Szklarczyk; Agnieszka Kiełkowska

doi:10.1007/978-1-0716-1335-1_11

Haploid and Doubled Haploid Plant Production in Brassica rapa L. subsp. pekinensis Via Microspore Culture

Methods Mol Biol. 2021:2288:181-199. doi: 10.1007/978-1-0716-1335-1_11.

Authors

Adela Adamus¹, Marek Szklarczyk¹, Agnieszka Kiełkowska²

Affiliations

¹ Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Krakow, Poland.
² Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Krakow, Poland. a.kielkowska@urk.edu.pl.

PMID: 34270012
DOI: 10.1007/978-1-0716-1335-1_11

Abstract

The production of haploid and doubled haploid plants is a biotechnological tool that shortens the breeding process of new cultivars in many species. Doubled haploid plants are homozygous at every locus and they can be utilized as parents to produce F1 hybrids. In this chapter, we describe a protocol for the production of doubled haploid plants in Brassica rapa L. subsp. pekinensis using androgenesis induced by isolated microspore cultures.

Keywords: Brassica; Doubled haploid; Heat shock; Microspores; Pekinensis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acclimatization / genetics
Brassica rapa / genetics*
Brassica rapa / growth & development*
Brassica rapa / physiology
Crops, Agricultural / genetics
Crops, Agricultural / growth & development
Crops, Agricultural / physiology
Culture Media / chemistry
DNA, Plant / genetics
Diploidy
Glucose-6-Phosphate Isomerase / genetics
Haploidy
Homozygote
Molecular Biology / methods
Plant Breeding / methods*
Pollen / genetics
Pollen / growth & development
Polymerase Chain Reaction
Regeneration / genetics
Tissue Culture Techniques

Substances

Culture Media
DNA, Plant
Glucose-6-Phosphate Isomerase