An electrodiffusive neuron-extracellular-glia model for exploring the genesis of slow potentials in the brain

PLoS Comput Biol. 2021 Jul 16;17(7):e1008143. doi: 10.1371/journal.pcbi.1008143. eCollection 2021 Jul.

Abstract

Within the computational neuroscience community, there has been a focus on simulating the electrical activity of neurons, while other components of brain tissue, such as glia cells and the extracellular space, are often neglected. Standard models of extracellular potentials are based on a combination of multicompartmental models describing neural electrodynamics and volume conductor theory. Such models cannot be used to simulate the slow components of extracellular potentials, which depend on ion concentration dynamics, and the effect that this has on extracellular diffusion potentials and glial buffering currents. We here present the electrodiffusive neuron-extracellular-glia (edNEG) model, which we believe is the first model to combine compartmental neuron modeling with an electrodiffusive framework for intra- and extracellular ion concentration dynamics in a local piece of neuro-glial brain tissue. The edNEG model (i) keeps track of all intraneuronal, intraglial, and extracellular ion concentrations and electrical potentials, (ii) accounts for action potentials and dendritic calcium spikes in neurons, (iii) contains a neuronal and glial homeostatic machinery that gives physiologically realistic ion concentration dynamics, (iv) accounts for electrodiffusive transmembrane, intracellular, and extracellular ionic movements, and (v) accounts for glial and neuronal swelling caused by osmotic transmembrane pressure gradients. The edNEG model accounts for the concentration-dependent effects on ECS potentials that the standard models neglect. Using the edNEG model, we analyze these effects by splitting the extracellular potential into three components: one due to neural sink/source configurations, one due to glial sink/source configurations, and one due to extracellular diffusive currents. Through a series of simulations, we analyze the roles played by the various components and how they interact in generating the total slow potential. We conclude that the three components are of comparable magnitude and that the stimulus conditions determine which of the components that dominate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology*
  • Animals
  • Brain / cytology
  • Brain / physiology
  • Computational Biology
  • Electric Stimulation*
  • Extracellular Space / physiology
  • Models, Neurological*
  • Neuroglia / physiology*
  • Neurons / physiology*

Grant support

This work was funded by the Research Council of Norway (Norges Forskningsråd: https://www.forskningsradet.no) via the BIOTEK2021 Digital Life project ‘DigiBrain’, grant no 248828 (received by GTE), and EU project (Horizon 2020) HBP SGA3 Grant agreement ID: 945539 (received by GTE). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.