A mitochondria-targeted fluorescent probe for real-time imaging SO2/H2O2

Yang Wang; Ru-Xing Chen; Rui Tian; Yan Li; Zongxia Guo; Ying Fang; Qi Zhang; Shaojin Chen; Kun-Peng Wang; Zhi-Qiang Hu

doi:10.1016/j.saa.2021.120134

A mitochondria-targeted fluorescent probe for real-time imaging SO₂/H₂O₂

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Dec 5:262:120134. doi: 10.1016/j.saa.2021.120134. Epub 2021 Jul 3.

Authors

Yang Wang¹, Ru-Xing Chen², Rui Tian³, Yan Li⁴, Zongxia Guo⁴, Ying Fang², Qi Zhang⁵, Shaojin Chen², Kun-Peng Wang², Zhi-Qiang Hu⁶

Affiliations

¹ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
² Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
³ School of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
⁴ College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
⁵ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: zhangqi@qust.edu.cn.
⁶ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: huzhiqiang@qust.edu.cn.

PMID: 34271238
DOI: 10.1016/j.saa.2021.120134

Abstract

Studies have shown that changes in the redox state of cells might be closely related to pathological and physiological processes. Sulfur dioxide and hydrogen peroxide, as a significant redox couple in living cells, are endogenously produced by cells. Here, we report a long-wavelength fluorescent probe to reversibly monitor sulfur dioxide and hydrogen peroxide. This probe (NBD) displayed high selectivity and sensitivity, which could be accumulated in mitochondria for real-time imaging of SO₂/H₂O₂. These results indicated that NBD would be an ideal tool for monitoring the redox cycle state in living cells.

Keywords: Hydrogen peroxide; Long-wavelength probe; Redox state; Reversible detection; Sulfur dioxide.

MeSH terms

Fluorescent Dyes*
HeLa Cells
Humans
Hydrogen Peroxide*
Mitochondria
Optical Imaging
Sulfur Dioxide

Substances

Fluorescent Dyes
Sulfur Dioxide
Hydrogen Peroxide