Protective effects of Quercetin and Resveratrol on aging markers in kidney under high glucose condition: in vivo and in vitro analysis

Mol Biol Rep. 2021 Jul;48(7):5435-5442. doi: 10.1007/s11033-021-06550-3. Epub 2021 Jul 17.


Background: Resveratrol and Quercetin as powerful herbal antioxidant reduce kidney complications of diabetes. The purpose of this study was to evaluate the protective effects of Quercetin and Resveratrol on aging markers in high glucose condition.

Methods and results: In this study, HEK-293 cells and kidney tissue of male Wistar rats were used in hyperglycemia condition. Quercetin and Resveratrol for cell culture and in vivo evaluation were used. Amount of methylglyoxal and the gene and protein expression of the Senescence Marker Protein30 (SMP30) were measured using qRT-PCR and ELISA, respectively. High glucose condition in kidney cell and tissue caused increase in glyoxal amount compared to control sample. Treatment with Resveratrol and/or Quercetin significantly decreased the amount of methylglyoxal in a dose-dependent manner. SMP30 expression significantly decreased compare to control group while treatment with Resveratrol and/or Quercetin considerably increased expression level of the protein. Six weeks treatment with Quercetin and Resveratrol had more beneficial effects on aging markers compared with three weeks.

Conclusions: According to the results, it can be concluded that resveratrol and quercetin, as potent herbal antioxidants, may have significant effects in reducing the complications of diabetes in kidneys, as well as preventing the aging of the kidney cells.

Keywords: Aging; Diabetes; Kidney; Quercetin; Resveratrol.

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Biomarkers*
  • Blood Glucose / drug effects*
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / metabolism
  • Carboxylic Ester Hydrolases / genetics
  • Carboxylic Ester Hydrolases / metabolism
  • Cellular Senescence / drug effects*
  • Diabetic Nephropathies / drug therapy
  • Diabetic Nephropathies / etiology
  • Diabetic Nephropathies / metabolism
  • Diabetic Nephropathies / pathology
  • Disease Models, Animal
  • Glucose / metabolism
  • Glucose / pharmacology
  • HEK293 Cells
  • Humans
  • Immunohistochemistry
  • Kidney / drug effects*
  • Kidney / metabolism*
  • Oxidative Stress / drug effects
  • Pyruvaldehyde / metabolism
  • Quercetin / pharmacology*
  • Rats
  • Resveratrol / pharmacology*


  • Antioxidants
  • Biomarkers
  • Blood Glucose
  • Calcium-Binding Proteins
  • Pyruvaldehyde
  • Quercetin
  • Carboxylic Ester Hydrolases
  • Rgn protein, rat
  • Glucose
  • Resveratrol