Dynamin regulates L cell secretion in human gut

Mol Cell Endocrinol. 2021 Sep 15:535:111398. doi: 10.1016/j.mce.2021.111398. Epub 2021 Jul 15.


Background: The mechanochemical enzyme dynamin mediates endocytosis and regulates neuroendocrine cell exocytosis. Enteroendocrine L cells co-secrete the anorectic gut hormones glucagon-like peptide 1 (GLP-1) and peptide YY (PYY) postprandially and is a potential therapeutic target for metabolic diseases. In the present study, we aimed to determine if dynamin is implicated in human L cell secretion.

Methods: Western blot was performed on the murine L cell line GLUTag. Static incubation of human colonic mucosae with activators and inhibitors of dynamin was carried out. GLP-1 and PYY contents of the secretion supernatants were assayed using ELISA.

Results and conclusion: s: Both dynamin I and II are expressed in GLUTag cells. The dynamin activator Ryngo 1-23 evoked significant GLP-1 and PYY release from human colonic mucosae while the dynamin inhibitor Dynole 3-42 significantly inhibited release triggered by known L cell secretagogues. Thus, the cell signaling regulator dynamin is able to bi-directionally regulate L cell hormone secretion in the human gut and may represent a novel target for gastrointestinal-targeted metabolic drug development.

Keywords: Dynamin; Enteroendocrine cells; GLP-1; Gut hormones; PYY.

MeSH terms

  • Adult
  • Aged
  • Animals
  • Cells, Cultured
  • Culture Media / chemistry
  • Cyanoacrylates / pharmacology
  • Dynamin I / metabolism*
  • Dynamin II / metabolism*
  • Enteroendocrine Cells / cytology*
  • Enteroendocrine Cells / drug effects
  • Enteroendocrine Cells / metabolism
  • Female
  • Glucagon-Like Peptide 1 / metabolism*
  • Humans
  • Indoles / pharmacology
  • Intestinal Mucosa / cytology*
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism
  • L Cells
  • Male
  • Mice
  • Middle Aged
  • Peptide YY / metabolism*
  • Tyrphostins / pharmacology


  • Culture Media
  • Cyanoacrylates
  • Indoles
  • Ryngo 1-23
  • Tyrphostins
  • dynole 34-2
  • Peptide YY
  • Glucagon-Like Peptide 1
  • Dynamin I
  • DNM2 protein, human
  • Dynamin II