Obesogenic and developmental effects of TBT on the gene expression of juvenile Japanese medaka (Oryzias latipes)

Kun Chen; Naoto Iwasaki; Xuchun Qiu; Hai Xu; Yuki Takai; Kosuke Tashiro; Yohei Shimasaki; Yuji Oshima

doi:10.1016/j.aquatox.2021.105907

Obesogenic and developmental effects of TBT on the gene expression of juvenile Japanese medaka (Oryzias latipes)

Aquat Toxicol. 2021 Aug:237:105907. doi: 10.1016/j.aquatox.2021.105907. Epub 2021 Jul 2.

Authors

Kun Chen¹, Naoto Iwasaki², Xuchun Qiu³, Hai Xu⁴, Yuki Takai², Kosuke Tashiro⁵, Yohei Shimasaki², Yuji Oshima⁶

Affiliations

¹ Institute of Environmental Health and Ecological Security, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China; Laboratory of Marine Environmental Science, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan.
² Laboratory of Marine Environmental Science, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan.
³ Institute of Environmental Health and Ecological Security, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China; Laboratory of Marine Environmental Science, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan. Electronic address: xuchunqiu@ujs.edu.cn.
⁴ Institute of Environmental Health and Ecological Security, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
⁵ Laboratory of Molecular Gene Technology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan.
⁶ Laboratory of Marine Environmental Science, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan; Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa 920-1192, Japan. Electronic address: yoshima@agr.kyushu-u.ac.jp.

PMID: 34274867
DOI: 10.1016/j.aquatox.2021.105907

Abstract

The widely used antifoulant tributyltin chloride (TBT) is highly toxic to aquatic organisms. In the present study, four-week-old Japanese medaka (Oryzias latipes) juveniles were orally exposed to TBT at 1 and 10 ng/g bw/d for 1, 2, and 4 weeks, respectively. Half of the tested medaka juveniles showed bone morphology alterations in both 1 and 10 ng/g bw/d TBT 4-week exposure groups. Nile Red (NR) staining showed that the juveniles exposed to 1 ng/g bw/d TBT for 2 and 4 weeks had significantly enlarged adipocyte areas. The mRNA-Seq analysis indicated that 1 ng/g bw/d TBT exposure for 2 weeks affected bone morphology through developmental processes. The GO and KEGG analyses suggested that the adipogenic effect of TBT observed in this study may be induced by metabolic processes, oxidative phosphorylation, and fatty acid degradation and metabolism pathways. Therefore, both morphological observation and mRNA-Seq analysis showed obesogenic effects and developmental toxicity of TBT to juvenile Japanese medaka.

Keywords: Developmental toxicity; Japanese medaka; Obesogenic effect; Tributyltin chloride; mRNA-Seq.

MeSH terms

Animals
Gene Expression
Oryzias* / genetics
Water Pollutants, Chemical* / toxicity

Substances

Water Pollutants, Chemical