Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

Ermi Girsang; Chrismis N Ginting; I Nyoman Ehrich Lister; Kamila Yashfa Gunawan; Wahyu Widowati

doi:10.7717/peerj.11419

Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell

PeerJ. 2021 Jul 7:9:e11419. doi: 10.7717/peerj.11419. eCollection 2021.

Authors

Ermi Girsang^#¹, Chrismis N Ginting¹, I Nyoman Ehrich Lister¹, Kamila Yashfa Gunawan², Wahyu Widowati^#³

Affiliations

¹ Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia.
² Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia.
³ Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.

^# Contributed equally.

Abstract

Background: Skin aging is the most common dermatological problem caused by intrinsic and extrinsic factor, such as exposure to (ultraviolet) UV rays. Chlorogenic acid (CA) is a phenolic compound which is known for its antioxidant properties against oxidative stress.

Objective: This study investigates the antiaging and anti-inflammatory properties of CA on UV-induced skin fibroblast cells.

Methods: Anti-inflammatory properties of CA were assessed by measuring inflammatory-related proteins IL-1β and TNF-α, while antiaging properties of CA were assessed by measuring reactive oxygen species (ROS), apoptosis, live and necrotic cells, and COL-3 gene expression level.

Results: Treating UV-induced skin fibroblast cells with CA decreased the level of ROS, IL-1β, TNF-α, apoptotic cells, and necrotic cells and increased live cells and COL-3 gene expression.

Conclusion: CA has the potential as the protective compound against inflammation and aging by decreasing the level ROS, pro-inflammatory cytokines IL-1β and TNF-α, apoptotic cells, and necrotic cells and by increasing live cells and COL-3 gene expression.

Keywords: Anti-inflammatory; Antiaging; Apoptosis; Chlorogenic acid; Ultraviolet.

Grants and funding

This study was supported by The Ministry of Education, Culture, Research, and Technology, Indonesia for research grant of PDUPT (Penelitian Dasar Unggulan Perguruan Tinggi) 2021. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.