Effects of Kumaizasa (Sasa senanensis) Leaf Extract on Innate Immune Regulation in HEK293 Cells and Macrophages

Anticancer Res. 2021 Aug;41(8):4093-4100. doi: 10.21873/anticanres.15212.


Background/aim: We investigated the effect of Kumaizasa leaf extract (KLE) on innate immunity using the HEK293 and RAW 264.7 cell lines.

Materials and methods: KLE, lipopolysaccharides (LPS), or KLE with LPS were added to RAW 264.7 cells. The TNF-α and IL-1β mRNA expression was then quantified. The expression of MAPKs, NFĸB, TNF-α and IL-1β proteins was also quantified. In addition, KLE was added to HEK293 cells and the IL-8 concentration was measured.

Results: In RAW 264.7 cells, KLE increased the levels of TNF-α and IL-1β mRNA. By contrast, when KLE and LPS were added to RAW 264.7 cells, the increase in TNF-α and IL-1β mRNA was ameliorated. Similarly, the expression of JNK and ERK proteins was reduced. The addition of KLE to HEK293 cells induced IL-8 production.

Conclusion: Based on these results, a KLE-mediated mechanism may regulate immunity by suppressing the expression of JNK and ERK, which are involved in inflammatory signal transduction.

Keywords: HEK293; Kumaizasa; RAW 264.7; Sasa senanensis; c-Jun N-terminal kinase; extracellular signal-regulated kinase; interleukin-1β; toll-like receptor 2; toll-like receptor 4; tumor necrosis factor-α.

MeSH terms

  • Animals
  • Cytokines / genetics
  • Cytokines / immunology
  • Extracellular Signal-Regulated MAP Kinases / immunology
  • HEK293 Cells
  • Humans
  • Immunity, Innate / drug effects*
  • JNK Mitogen-Activated Protein Kinases / immunology
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects*
  • Macrophages / immunology
  • Mice
  • Plant Extracts / pharmacology*
  • Plant Leaves
  • RAW 264.7 Cells
  • Sasa*


  • Cytokines
  • Lipopolysaccharides
  • Plant Extracts
  • Extracellular Signal-Regulated MAP Kinases
  • JNK Mitogen-Activated Protein Kinases