Co-upgrading of ethanol-assisted depolymerized lignin: A new biological lignin valorization approach for the production of protocatechuic acid and polyhydroxyalkanoic acid

Bioresour Technol. 2021 Oct;338:125563. doi: 10.1016/j.biortech.2021.125563. Epub 2021 Jul 14.

Abstract

This study presents a promising biological co-upgrading of ethanol-assisted depolymerized lignin (EDL) into protocatechuic acid (PCA) and polyhydroxyalkanoic acid (PHA) without any separation process. A depolymerized alkali lignin containing various G-lignin-type monomers at a concentration of 77 mg/mL was used for co-upgrading. An engineered Pseudomonas putida KT2440 strain was constructed by knocking out the protocatechuate 3, 4-dioxygenase, expression of the formaldehyde utilization pathway, and the expression of aldehyde dehydrogenase to enhance the efficiency of the ethanol utilization pathway. The growth and production of value-added bioproducts have been promoted by the utilization of formaldehyde, resulted in 6.73 ± 0.26 mg/L of PCA with a 17.5% (w/w) yield of total lignin monomers, and 303.66 ± 26.75 mg/L of PHA with 21.26% (w/w) of dry cell weight from 0.5 mL EDL. Moreover, the ethanol solvent used for lignin depolymerization was also utilized along with depolymerized lignin for co-upgrading to value-added products.

Keywords: Co-upgrading; Ethanol utilization; Lignin valorization; Polyhydroxyalkanoic acid; Protocatechuic acid.

MeSH terms

  • Ethanol
  • Hydroxybenzoates
  • Lignin*
  • Pseudomonas putida*

Substances

  • Hydroxybenzoates
  • protocatechuic acid
  • Ethanol
  • Lignin