KLF7 Promotes Gastric Carcinogenesis Through Regulation of ANTXR1

Yuanchun Li; Qingdong Wang; DongWei Wang; Weihua Fu

doi:10.2147/CMAR.S308071

KLF7 Promotes Gastric Carcinogenesis Through Regulation of ANTXR1

Cancer Manag Res. 2021 Jul 12:13:5547-5557. doi: 10.2147/CMAR.S308071. eCollection 2021.

Authors

Yuanchun Li^#¹, Qingdong Wang^#², DongWei Wang², Weihua Fu³

Affiliations

¹ Department of General Surgery, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, People's Republic of China.
² Department of Anesthesiology, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, People's Republic of China.
³ Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China.

^# Contributed equally.

Abstract

Purpose: Elucidating the mechanism of gastric cancer progression is of great importance for the discovery of new therapy targets against gastric cancer. In this study, we investigated the function of Kruppel-like factor 7 (KLF7) in gastric cancer.

Methods: qPCR and Western blot were performed to determine the expression of ANTXR1 after KLF7 inhibition. CCK-8, colony formation, apoptosis analysis, cell cycle analysis and transwell assay were performed to determine KLF7 functions in cellular proliferation, migration, apoptosis and cell cycle. Tumour xenograft experiments were performed to examine cell growth in vivo.

Results: The results showed that KLF7 was upregulated in gastric cancer. The proliferation and migration of gastric cancer cells were suppressed by depletion of KLF7. In vivo tumour progression was also attenuated following the downregulation of KLF7. Meanwhile, overexpression of KLF7 promoted the proliferation and migration of gastric cancer cells. The results of the mechanistic analysis showed that KLF7 promoted gastric carcinogenesis via upregulation of ANTXR cell adhesion molecule 1 (ANTXR1).

Conclusion: Therefore, this study may provide a theoretical foundation for further clinical therapy of gastric cancer.

Keywords: ANTXR1; KLF7; gastric cancer; migration; proliferation.

Publication types

Retracted Publication

Grants and funding

This work was supported by the Jiamusi University doctoral special scientific research foundation (JMSUBZ2019-05) and Heilongjiang Traditional Chinese medicine authority Scientific Research Project (ZHY2020-177).