Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

Valérie Allasia; Benoit Industri; Michel Ponchet; Michaël Quentin; Bruno Favery; Harald Keller

doi:10.21769/BioProtoc.2844

Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

Bio Protoc. 2018 May 20;8(10):e2844. doi: 10.21769/BioProtoc.2844.

Authors

Valérie Allasia¹, Benoit Industri¹, Michel Ponchet¹, Michaël Quentin¹, Bruno Favery¹, Harald Keller¹

Affiliation

¹ INRA, Université Côte d'Azur, CNRS, Institut Sophia Agrobiotech, Sophia-Antipolis, France.

Abstract

Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA in plant tissues using a classical HPLC-based method.

Keywords: Arabidopsis thaliana; Defense hormone; HPLC; SA-glucoside; Salicylic acid.