In vitro Explant Cultures to Interrogate Signaling Pathways that Regulate Mouse Lung Development

Changfu Yao; Gianni Carraro; Barry R Stripp

doi:10.21769/BioProtoc.2852

In vitro Explant Cultures to Interrogate Signaling Pathways that Regulate Mouse Lung Development

Bio Protoc. 2018 May 20;8(10):e2852. doi: 10.21769/BioProtoc.2852.

Authors

Changfu Yao^{1

2}, Gianni Carraro^{1

2}, Barry R Stripp^{1

2}

Affiliations

¹ Lung Institute, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
² Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.

Abstract

Early mouse lung development, including specification of primordia, patterning of early endoderm and determination of regional progenitor cell fates, is tightly regulated. The ability to culture explanted embryonic lung tissue provides a tractable model to study cellular interactions and paracrine factors that regulate these processes. We provide up-to-date protocols for the establishment of this culture model and its application to investigate hedgehog signaling in the developing lung.

Keywords: Explant culture; In vitro; Mouse embryonic lung.