Quantifying Podocytes and Parietal Epithelial Cells in Human Urine Using Liquid-based Cytology and WT1 Immunoenzyme Staining

Hiroyuki Ohsaki; Toru Matsunaga; Taishi Fujita; Yasunori Tokuhara; Shingo Kamoshida; Tadashi Sofue

doi:10.21769/BioProtoc.2827

Quantifying Podocytes and Parietal Epithelial Cells in Human Urine Using Liquid-based Cytology and WT1 Immunoenzyme Staining

Bio Protoc. 2018 May 5;8(9):e2827. doi: 10.21769/BioProtoc.2827.

Authors

Hiroyuki Ohsaki¹, Toru Matsunaga², Taishi Fujita³, Yasunori Tokuhara⁴, Shingo Kamoshida¹, Tadashi Sofue⁵

Affiliations

¹ Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan.
² Department of Diagnostic Pathology, University Hospital, Faculty of Medicine, Kagawa University, Kagawa, Japan.
³ Department of Clinical Laboratory, Ehime General Health Care Association, Ehime, Japan.
⁴ Department of Medical Technology, Ehime Prefectural University of Health Sciences, Ehime, Japan.
⁵ Department of Cardiorenal and Cerebrovascular Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan.

Abstract

In glomerular disease, podocytes and parietal epithelial cells (PECs) are shed in the urine. Therefore, urinary podocytes and PECs are noninvasive biomarkers of glomerular disease. The purpose of this protocol is to employ immunocytochemistry to detect podocytes and PECs, using the WT1 antibody on liquid-based cytology slides.

Keywords: Crescent formation; Glomerular disease; Immunocytochemistry; Liquid-based cytology; Parietal epithelial cells; Podocytes; Urine; WT1.