Mitotic disassembly and reassembly of nuclear pore complexes

Trends Cell Biol. 2021 Dec;31(12):1019-1033. doi: 10.1016/j.tcb.2021.06.011. Epub 2021 Jul 19.


Nuclear pore complexes (NPCs) are huge protein assemblies within the nuclear envelope (NE) that serve as selective gates for macromolecular transport between nucleus and cytoplasm. When higher eukaryotic cells prepare for division, they rapidly disintegrate NPCs during NE breakdown such that nuclear and cytoplasmic components mix to enable the formation of a cytoplasmic mitotic spindle. At the end of mitosis, reassembly of NPCs is coordinated with the establishment of the NE around decondensing chromatin. We review recent progress on mitotic NPC disassembly and reassembly, focusing on vertebrate cells. We highlight novel mechanistic insights into how NPCs are rapidly disintegrated into conveniently reusable building blocks, and put divergent models of (post-)mitotic NPC assembly into a spatial and temporal context.

Keywords: mitosis; nuclear envelope breakdown; nuclear pore complex; nuclear reassembly.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Nucleus / metabolism
  • Humans
  • Mitosis
  • Nuclear Envelope / metabolism
  • Nuclear Pore Complex Proteins* / metabolism
  • Nuclear Pore* / metabolism


  • Nuclear Pore Complex Proteins