8-Hydroxy-2'-Deoxyguanosine as an Oxidative Stress Marker in Insomnia

Bull Exp Biol Med. 2021 Jul;171(3):384-387. doi: 10.1007/s10517-021-05233-0. Epub 2021 Jul 23.

Abstract

We performed comparative analysis of 8-hydroxy-2'-deoxyguanosine in women in different climax stages with and without insomnia. The study involved 90 women aged 45 to 60 years divided into perimenopausal (n=30) and postmenopausal (n=60) groups. After questioning using special sleep questionnaires (Insomnia Severity Index, Epworth Sleepiness Scale, Munich Chronotype Questionnaire), the groups were divided into subgroups with insomnia and without it (control). 8-Hydroxy-2'-deoxyguanosine was assayed in blood serum by ELISA. The higher levels of 8-hydroxy-2'-deoxyguanosine in postmenopausal women with insomnia in comparison with the control and perimenopausal patients (p<0.05) attested to oxidative DNA damage in this cohort of patients.

Keywords: 8-hydroxy-2’-deoxyguanosine; insomnia; oxidative stress; perimenopause; postmenopause.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine / blood*
  • Biomarkers / blood
  • Case-Control Studies
  • DNA Damage*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Middle Aged
  • Oxidative Stress
  • Perimenopause / blood*
  • Perimenopause / psychology
  • Postmenopause / blood*
  • Postmenopause / psychology
  • Severity of Illness Index
  • Sleep / physiology
  • Sleep Initiation and Maintenance Disorders / blood*
  • Sleep Initiation and Maintenance Disorders / diagnosis
  • Sleep Initiation and Maintenance Disorders / physiopathology
  • Sleep Initiation and Maintenance Disorders / psychology
  • Surveys and Questionnaires

Substances

  • Biomarkers
  • 8-Hydroxy-2'-Deoxyguanosine