Large-Scale Differentiation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture

Nagako Sougawa; Shigeru Miyagawa; Yoshiki Sawa

doi:10.1007/978-1-0716-1484-6_3

Large-Scale Differentiation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture

Methods Mol Biol. 2021:2320:23-27. doi: 10.1007/978-1-0716-1484-6_3.

Authors

Nagako Sougawa¹, Shigeru Miyagawa¹, Yoshiki Sawa²

Affiliations

¹ Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan.
² Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan. sawa-p@surg1.med.osaka-u.ac.jp.

PMID: 34302644
DOI: 10.1007/978-1-0716-1484-6_3

Abstract

Regenerative medicine using human-induced pluripotent stem cells (hiPSCs) is a promising approach to treat heart failure. However, a large number of cells are required to achieve the desired therapeutic effect. The stirring-type suspension culture method allows a large-scale production of hiPSC-derived cardiomyocytes (more than 1 × 10⁸ cells/100 mL), leading to a stable cell supply. Here, we describe a method to scale-up hiPSC-derived cardiomyocyte production with a high differentiation efficiency.

Keywords: Cardiomyocytes; Differentiation; Large-scale; Stirring-type suspension culture; hiPSCs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods
Cell Differentiation / physiology*
Cells, Cultured
Humans
Induced Pluripotent Stem Cells / cytology*
Mice
Myocytes, Cardiac / cytology*