Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells

Daisuke Sasaki; Katsuhisa Matsuura; Tatsuya Shimizu

doi:10.1007/978-1-0716-1484-6_16

Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells

Methods Mol Biol. 2021:2320:161-170. doi: 10.1007/978-1-0716-1484-6_16.

Authors

Daisuke Sasaki¹, Katsuhisa Matsuura^{1

2}, Tatsuya Shimizu³

Affiliations

¹ Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
² Department of Cardiology, Tokyo Women's Medical University, Tokyo, Japan.
³ Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan. shimizu.tatsuya@twmu.ac.jp.

PMID: 34302657
DOI: 10.1007/978-1-0716-1484-6_16

Abstract

Recent advances in stem cell technologies and tissue engineering are enabling the fabrication of dynamically beating cardiac tissues from human induced pluripotent stem cells. These engineered human cardiac tissues are expected to be used for cardiac regenerative therapies, in vitro drug testing, and pathological investigations. Here we describe the method to fabricate engineered cardiac tissues from human induced pluripotent stem cell-derived cardiomyocytes and to measure the contractile force.

Keywords: Cardiomyocytes; Contractile force; In vitro drug testing; Temperature-responsive cell culture dish; Tissue engineering; iPS cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Humans
Induced Pluripotent Stem Cells / cytology*
Muscle Contraction / physiology*
Myocytes, Cardiac / cytology*
Tissue Engineering / methods