Transplantation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in a Mouse Myocardial Infarction Model

Methods Mol Biol. 2021:2320:285-293. doi: 10.1007/978-1-0716-1484-6_24.

Abstract

Myocardial infarction is caused by a lack of oxygen due to blockage of a coronary artery and is a common cause of heart failure. Despite therapeutic advances, the prognosis of patients with heart failure is poor. One of the reasons is that present therapeutic approaches do not restore the loss of cardiac tissue. Stem cell-based therapies have the potential to regenerate the myocardium, and numerous studies using stem cells have shown improved cardiac function and reduced infarct size. In this chapter, we describe our methodology for transplanting human induced pluripotent stem cell-derived cardiomyocytes into immunodeficient mouse hearts with myocardial infarction.

Keywords: Cardiomyocyte transplantation; Human induced pluripotent stem cell-derived cardiomyocytes; Myocardial infarction; Regeneration therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Heart / physiology
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Injections, Intramuscular
  • Ligation
  • Male
  • Mice
  • Mice, Inbred NOD
  • Myocardial Infarction / therapy
  • Myocytes, Cardiac / transplantation*
  • Regeneration
  • Respiration, Artificial / methods
  • Respiration, Artificial / veterinary
  • Thoracotomy / methods
  • Thoracotomy / veterinary