A sensitive molecularly imprinted electrochemical aptasensor for highly specific determination of melamine

Chenhong Yu; Li Li; Yaping Ding; Huajie Liu; Hanyue Cui; Fenfen Zhang; Jiaxin Lin; Yingchun Duan

doi:10.1016/j.foodchem.2021.130202

A sensitive molecularly imprinted electrochemical aptasensor for highly specific determination of melamine

Food Chem. 2021 Nov 30:363:130202. doi: 10.1016/j.foodchem.2021.130202. Epub 2021 Jun 8.

Authors

Chenhong Yu¹, Li Li², Yaping Ding³, Huajie Liu⁴, Hanyue Cui¹, Fenfen Zhang⁵, Jiaxin Lin¹, Yingchun Duan¹

Affiliations

¹ Department of Chemistry, College of Sciences, Shanghai University, Shanghai 200444, PR China.
² Department of Chemistry, College of Sciences, Shanghai University, Shanghai 200444, PR China. Electronic address: lilidu@shu.edu.cn.
³ Department of Chemistry, College of Sciences, Shanghai University, Shanghai 200444, PR China. Electronic address: wdingyp@sina.com.
⁴ School of Chemical Science and Engineering, Tongji University, Shanghai 200092, PR China. Electronic address: liuhuajie@tongji.edu.cn.
⁵ School of Water Conservancy and Environment, University of Jinan, Jinan 250022, PR China.

PMID: 34304106
DOI: 10.1016/j.foodchem.2021.130202

Abstract

An electrochemical aptamer sensor based on gold nanoparticles (AuNPs) was developed for highly specific sensing of melamine (MEL), which combines molecularly imprinted polymers (MIPs) and aptamers. AuNPs were synthesized by simple reduction of sodium citrate and characterized by transmission electron microscopy. The MIP membranes with particular recognition sites were formed by electropolymerization of dopamine (DA) with polythymine (poly T) aptamers as functional monomers and melamine as template molecules. Under optimal experimental conditions, this molecularly imprinted electrochemical aptamer sensor (MIEAS) exhibits a linear relationship between 10^-12 M and 10^-4 M for detecting MEL with the detection limit of 6.7 × 10^-13 M. Moreover, this sensor displays excellent selectivity, reproducibility and stability. The milk samples analysis has confirmed the potential application of this MIEAS to quantitative detection of melamine.

Keywords: Aptamer; AuNPs; Melamine; Molecularly imprinted.

MeSH terms

Biosensing Techniques*
Electrochemical Techniques
Electrodes
Gold
Limit of Detection
Metal Nanoparticles*
Molecular Imprinting*
Reproducibility of Results
Triazines

Substances

Triazines
Gold
melamine