Indole-Terpenoids With Anti-inflammatory Activities From Penicillium sp. HFF16 Associated With the Rhizosphere Soil of Cynanchum bungei Decne

Guojun Pan; Yanfen Zhao; Shuang Ren; Fengyang Liu; Qicai Xu; Weibin Pan; Tongtao Yang; Mingtian Yang; Xinru Zhang; Chuanyue Peng; Gangping Hao; Fandong Kong; Liman Zhou; Na Xiao

doi:10.3389/fmicb.2021.710364

Indole-Terpenoids With Anti-inflammatory Activities From Penicillium sp. HFF16 Associated With the Rhizosphere Soil of Cynanchum bungei Decne

Front Microbiol. 2021 Jul 9:12:710364. doi: 10.3389/fmicb.2021.710364. eCollection 2021.

Authors

Guojun Pan¹, Yanfen Zhao¹, Shuang Ren¹, Fengyang Liu¹, Qicai Xu¹, Weibin Pan², Tongtao Yang³, Mingtian Yang¹, Xinru Zhang¹, Chuanyue Peng¹, Gangping Hao¹, Fandong Kong², Liman Zhou², Na Xiao^{3

4}

Affiliations

¹ College of Life Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, China.
² Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning, China.
³ State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agriculture University, Tai'an, China.
⁴ State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.

Abstract

Four new indole-terpenoids (1-4) named encindolene A, 18-O-methyl-encindolene A, encindolene B, and encindolene C, as well as three known analogs (5-7), were isolated from the fungus Penicillium sp. HFF16 from the rhizosphere soil of Cynanchum bungei Decne. The structures of compounds including absolute configurations were elucidated by spectroscopic data and electronic circular dichroism (ECD) analysis. Anti-inflammatory activity evaluation revealed that compounds 1-7 inhibit the production of nitric oxide with IC₅₀ values of 79.4, 49.7, 81.3, 40.2, 86.7, 90.1, and 54.4 μM, respectively, and decrease the levels of tumor necrosis factor-α, interleukin-6 contents in lipopolysaccharide-induced RAW264.7 macrophages.

Keywords: Cynanchum bungei Decne; Penicillium sp. HFF16; anti-inflammatory activity; fungus; indole-terpenoids.