MFP1/MSD-1 and MFP2/NSPH-2 co-localize with MSP during C. elegans spermatogenesis

MicroPubl Biol. 2021 Jul 22;2021:10.17912/micropub.biology.000427. doi: 10.17912/micropub.biology.000427. eCollection 2021.


Until recently, the only verified component of Fibrous Bodies (FBs) within Caenorhabditis elegans spermatocytes was the Major Sperm Protein (MSP), a nematode-specific cytoskeletal element. Earlier studies in the pig parasite Ascaris suum had identified accessory proteins that facilitate MSP polymerization and depolymerization within the pseudopod of crawling spermatozoa. In this study, we show that C. elegans homologs of the two Ascaris accessory proteins MFP1 and MFP2 co-localize with MSP in both the pseudopods of C. elegans sperm and the FBs of C. elegans spermatocytes.