High yield recovery of 2,3-butanediol from fermented broth accumulated on xylose rich sugarcane bagasse hydrolysate using aqueous two-phase extraction system

Bioresour Technol. 2021 Oct:337:125463. doi: 10.1016/j.biortech.2021.125463. Epub 2021 Jun 26.

Abstract

Downstream processing of chemicals obtained from fermentative route is challenging and cost-determining factor of any bioprocess. 2,3-Butanediol (BDO) is a promising chemical building block with myriad applications in the polymer, food, pharmaceuticals, and fuel sector. The current study focuses on the recovery and purification of BDO produced (68.2 g/L) from detoxified xylose-rich sugarcane bagasse hydrolysate by a mutant strain of Enterobacter ludwigii. Studies involving screening and optimization of aqueous-two phase system (ATPS) revealed that 30% w/v (NH4)2SO4 addition to clarified fermented broth facilitated BDO extraction in isopropanol (0.5 v/v), with maximum recovery and partition coefficient being 97.9 ± 4.6% and 45.5 ± 3.5, respectively. The optimized protocol was repeated with unfiltered broth containing 68.2 g/L BDO, cell biomass, and unspent protein, which led to the partitioning of 66.7 g/L BDO, 2.0 g/L xylose and 9.0 g/L acetic acid into organic phase with similar BDO recovery (97%) and partition coefficient (45).

Keywords: 2,3-Butanediol; Ammonium sulphate, Isopropanol; Aqueous two-phase system; Enterobacter ludwigii; Xylose.

MeSH terms

  • Butylene Glycols
  • Cellulose
  • Enterobacter
  • Fermentation
  • Saccharum*
  • Xylose*

Substances

  • Butylene Glycols
  • 2,3-butylene glycol
  • Cellulose
  • bagasse
  • Xylose

Supplementary concepts

  • Enterobacter ludwigii