RNA sequencing identifies global transcriptional changes in peripheral CD4+ cells during active oesophagitis and following epicutaneous immunotherapy in eosinophilic oesophagitis

Melanie A Ruffner; Zhe Zhang; Kelly Maurer; Amanda B Muir; Antonella Cianferoni; Kathleen E Sullivan; Jonathan M Spergel

doi:10.1002/cti2.1314

RNA sequencing identifies global transcriptional changes in peripheral CD4⁺ cells during active oesophagitis and following epicutaneous immunotherapy in eosinophilic oesophagitis

Clin Transl Immunology. 2021 Jul 22;10(7):e1314. doi: 10.1002/cti2.1314. eCollection 2021.

Authors

Melanie A Ruffner^{1

2}, Zhe Zhang³, Kelly Maurer¹, Amanda B Muir^{2

4}, Antonella Cianferoni^{1

2}, Kathleen E Sullivan^{1

2}, Jonathan M Spergel^{1

2}

Affiliations

¹ Division of Allergy and Immunology The Children's Hospital of Philadelphia Philadelphia PA USA.
² Department of Pediatrics The Perelman School of Medicine at University of Pennsylvania Philadelphia PA USA.
³ Department of Biomedical and Health Informatics The Children's Hospital of Philadelphia Philadelphia PA USA.
⁴ Division of Gastroenterology, Hepatology, and Nutrition The Children's Hospital of Philadelphia Philadelphia PA USA.

Abstract

Objective: There are no disease-modifying therapies for the treatment of eosinophilic oesophagitis (EoE), which is driven by non-IgE-mediated allergic inflammation. A recent clinical trial of milk epicutaneous immunotherapy (EPIT) has shown initial promise, with 47% of treated EoE patients tolerating milk without recurrence of disease. Mechanisms of EPIT in EoE have not been studied in humans. Here, we identify transcriptional changes in the peripheral CD4⁺ T-cell compartment during active EoE and following EPIT.

Methods: RNA isolation, sequencing and integrative data analysis were performed on peripheral CD4⁺ T cells isolated from 15 of 20 patients enrolled in a clinical trial of EPIT for EoE. Gene expression changes in peripheral CD4⁺ T cells were examined during diet therapy and following trial of milk antigen EPIT.

Results: We identify 244 differentially expressed genes in peripheral blood CD4⁺ cells of EoE patients consuming versus those eliminating milk, and 129 DEGs in CD4⁺ cells were isolated after EPIT versus after placebo (FDR ≤ 0.05). Gene set enrichment analysis identifies enrichment of hallmark interferon-α and interferon-γ response pathways in peripheral CD4⁺ T cells from EoE patients during active disease on a milk-containing diet. We demonstrate overlap of this gene signature with the altered gene expression signature seen in EoE patient biopsy tissue. EPIT therapy response is associated with significant enrichment in pathways related to T-cell receptor signalling (P = 1.16 × 10^-14), antigen presentation and costimulation, and cytokine signalling (P = 1.11 × 10^-16), as well as upregulation of genes associated with regulatory T-cell function.

Conclusions: EoE is associated with distinct global transcriptional changes in CD4⁺ T cells, one feature of which is an IFN response signature. Clinically favorable response to EPIT is likely multifactorial but is associated with a distinct transcriptional profile in peripheral CD4⁺ cells supporting the hypothesis that EPIT alters peripheral CD4⁺ responses in EoE patients.

Keywords: CD4‐positive lymphocytes; RNA sequencing; eosinophilic oesophagitis; food allergy; immunotherapy; interferon response.

Abstract

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