The human melanoma proteome atlas-Defining the molecular pathology

Clin Transl Med. 2021 Jul;11(7):e473. doi: 10.1002/ctm2.473.

Abstract

The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in-depth histopathology coupled to proteome characterization. The protein levels and localization were determined for a broad spectrum of diverse, surgically isolated melanoma tumors originating from multiple body locations. More than 15,500 proteoforms were identified by mass spectrometry, from which chromosomal and subcellular localization was annotated within both primary and metastatic melanoma. The data generated by global proteomic experiments covered 72% of the proteins identified in the recently reported high stringency blueprint of the human proteome. This study contributes to the NIH Cancer Moonshot initiative combining detailed histopathological presentation with the molecular characterization for 505 melanoma tumor samples, localized in 26 organs from 232 patients.

Keywords: heterogeneity; histopathology; metastatic malignant melanoma; proteogenomics; subcellular localization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Line, Tumor
  • Chromatography, High Pressure Liquid
  • Female
  • Humans
  • Male
  • Melanoma / metabolism
  • Melanoma / pathology*
  • Middle Aged
  • Proteome / analysis*
  • Proteomics / methods*
  • Skin Neoplasms / metabolism
  • Skin Neoplasms / pathology*
  • Tandem Mass Spectrometry
  • Young Adult

Substances

  • Proteome

Supplementary concepts

  • Melanoma, Cutaneous Malignant