RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells

Methods Mol Biol. 2021;2319:25-30. doi: 10.1007/978-1-0716-1480-8_3.

Abstract

Fluid shear stress (FSS) is an important mechanical force that regulates endothelial and vascular functions in the blood and lymphatic vasculature. RNA-sequencing (RNA-Seq), an emerging next-generation sequencing (NGS) technique, facilitates us to quantify the global gene expression levels and, thereby, provides us with a powerful tool to study the transcriptional reprograming in the cells. Here, we describe the RNA-Seq procedures and the subsequent bioinformatic analysis to understand the transcriptome regulation by FSS in lymphatic endothelial cells (LECs).

Keywords: Fluid shear stress; Lymphatic endothelial cells; RNA-sequencing; Transcriptome.

MeSH terms

  • Cells, Cultured
  • Endothelial Cells / metabolism*
  • Gene Expression Profiling / methods*
  • Humans
  • RNA-Seq
  • Stress, Mechanical
  • Stress, Physiological / genetics*
  • Transcriptome / genetics*