Fourier Multiplexed Fluorescence Lifetime Imaging

Leilei Peng

doi:10.1007/978-1-0716-1593-5_11

Fourier Multiplexed Fluorescence Lifetime Imaging

Methods Mol Biol. 2021:2350:157-172. doi: 10.1007/978-1-0716-1593-5_11.

Author

Leilei Peng¹

Affiliation

¹ Wyant College of Optical Science, University of Arizona, Tucson, AZ, USA. lpeng@optics.arizona.edu.

PMID: 34331285
DOI: 10.1007/978-1-0716-1593-5_11

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a widely used functional imaging method in bioscience. Fourier multiplexed FLIM (FmFLIM), a frequency-domain lifetime measurement method, explores the principle of Fourier (frequency) multiplexing to achieve parallel lifetime detection on multiple fluorescence labels. Combining FmFLIM with a confocal scanning microscope allows multiplexed 3D lifetime imaging of cells and tissues. FmFLIM can also be integrated with the scanning laser tomography imaging method to perform 3D multiplex lifetime imaging of whole embryos and thick tissues.

Keywords: Förster resonance energy transfer; Lifetime imaging; Multiplexed imaging.

MeSH terms

Algorithms
Data Analysis
Fluorescence Resonance Energy Transfer / methods*
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Microscopy, Confocal / methods
Microscopy, Fluorescence, Multiphoton / methods*
Optical Imaging / methods*
Tomography, Optical / methods