Indirect evidence that anoxia exposure and cold acclimation alter transarcolemmal Ca2+ flux in the cardiac pacemaker, right atrium and ventricle of the red-eared slider turtle (Trachemys scripta)

Abstract

We indirectly assessed if altered transarcolemmal Ca²⁺ flux accompanies the decreased cardiac activity displayed by Trachemys scripta with anoxia exposure and cold acclimation. Turtles were first acclimated to 21 °C or 5 °C and held under normoxic (21N; 5N) or anoxic conditions (21A; 5A). We then compared the response of intrinsic heart rate (f_H) and maximal developed force of spontaneously contracting right atria (F_max,RA), and maximal developed force of isometrically-contracting ventricular strips (F_max,V), to Ni²⁺ (0.1-10 mM), which respectively blocks T-type Ca²⁺ channels, L-type Ca²⁺ channels and the Na⁺-Ca²⁺-exchanger at the low, intermediate and high concentrations employed. Dose-response curves were established in simulated in vivo normoxic (Sim Norm) or simulated in vivo anoxic extracellular conditions (Sim Anx; 21A and 5A preparations). Ni²⁺ decreased intrinsic f_H, F_max,RA and F_max,V of 21N tissues in a concentration-dependent manner, but the responses were blunted in 21A tissues in Sim Norm. Similarly, dose-response curves for F_max,RA and F_max,V of 5N tissues were right-shifted, whereas anoxia exposure at 5 °C did not further alter the responses. The influence of Sim Anx was acclimation temperature-, cardiac chamber- and contractile parameter-dependent. Combined, the findings suggest that: (1) reduced transarcolemmal Ca²⁺ flux in the cardiac pacemaker is a potential mechanism underlying the slowed intrinsic f_H of anoxic turtles at 21 °C, but not 5 °C, (2) a downregulation of transarcolemmal Ca²⁺ flux may aid cardiac anoxia survival at 21 °C and prime the turtle myocardium for winter anoxia and (3) confirm that altered extracellular conditions with anoxia exposure can modify turtle cardiac transarcolemmal Ca²⁺ flux.

Keywords: Anoxia; Cardiac pacemaker; Cardiac regulation; Ion channels; Myocardial contractile properties; Red-eared slider; Temperature.