O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an "O-Follow-N" rule

Wenmin Tian; Delin Li; Nan Zhang; Guijie Bai; Kai Yuan; Haixia Xiao; Feng Gao; Yang Chen; Catherine C L Wong; George Fu Gao

doi:10.1038/s41422-021-00545-2

O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an "O-Follow-N" rule

Cell Res. 2021 Oct;31(10):1123-1125. doi: 10.1038/s41422-021-00545-2. Epub 2021 Aug 2.

Authors

Wenmin Tian^#^{1

2}, Delin Li^#³, Nan Zhang^#^{1

2}, Guijie Bai^#^{3

4}, Kai Yuan^#^{3

4}, Haixia Xiao³, Feng Gao⁵, Yang Chen^{6

7}, Catherine C L Wong^{8

9

10

11

12}, George Fu Gao^{13

14}

Affiliations

¹ Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University, Beijing, China.
² School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
³ Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China.
⁴ Shanxi Academy of Advanced Research and Innovation, Taiyuan, Shanxi, China.
⁵ Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China. gaofeng@tib.cas.cn.
⁶ Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University, Beijing, China. chenyang1816185048@bjmu.edu.cn.
⁷ School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. chenyang1816185048@bjmu.edu.cn.
⁸ Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University, Beijing, China. catherine_wong@bjmu.edu.cn.
⁹ School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. catherine_wong@bjmu.edu.cn.
¹⁰ Peking-Tsinghua Center for Life Sciences, Beijing, China. catherine_wong@bjmu.edu.cn.
¹¹ Peking University First Hospital, Beijing, China. catherine_wong@bjmu.edu.cn.
¹² Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, China. catherine_wong@bjmu.edu.cn.
¹³ CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. gaof@im.ac.cn.
¹⁴ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, China. gaof@im.ac.cn.

^# Contributed equally.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
COVID-19 / pathology
COVID-19 / virology
Chromatography, High Pressure Liquid
Glycopeptides / analysis
Glycopeptides / chemistry
Glycopeptides / metabolism
Glycosylation
HEK293 Cells
Humans
SARS-CoV-2 / isolation & purification
SARS-CoV-2 / metabolism*
Spike Glycoprotein, Coronavirus / chemistry
Spike Glycoprotein, Coronavirus / genetics
Spike Glycoprotein, Coronavirus / metabolism*
Tandem Mass Spectrometry

Substances

Glycopeptides
Spike Glycoprotein, Coronavirus
spike protein, SARS-CoV-2