What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes

Jorge Monteford; Terry W Bilverstone; Patrick Ingle; Sheryl Philip; Sarah A Kuehne; Nigel P Minton

doi:10.1016/j.anaerobe.2021.102422

What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes

Anaerobe. 2021 Oct:71:102422. doi: 10.1016/j.anaerobe.2021.102422. Epub 2021 Jul 31.

Authors

Jorge Monteford¹, Terry W Bilverstone², Patrick Ingle², Sheryl Philip³, Sarah A Kuehne⁴, Nigel P Minton⁵

Affiliations

¹ Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, NG7 2RD, UK.
² Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and the University of Nottingham, Nottingham, NG7 2RD, UK.
³ Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, NG7 2RD, UK; Biocatalysts Limited, Unit 1, Cefn Coed, Parc Nantgarw, Cardiff, CF15 7QQ, UK.
⁴ Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, NG7 2RD, UK; Oral Microbiology Group, School of Dentistry and Institute of Microbiology and Infection, College of Medical and Dental Sciences, The University of Birmingham, Birmingham, B5 7EG, UK.
⁵ Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and the University of Nottingham, Nottingham, NG7 2RD, UK. Electronic address: nigel.minton@nottingham.ac.uk.

Abstract

Clostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes.

Keywords: Biofilm; Clostridioides difficile R20291; Conjugation; Genomic variation; Motility; Toxin production.

MeSH terms

Clostridioides / classification
Clostridioides / genetics*
Clostridioides / isolation & purification
Clostridioides difficile / classification
Clostridioides difficile / genetics
Clostridioides difficile / isolation & purification
Clostridium Infections / microbiology*
Genome, Bacterial
Humans
Phylogeny
Polymorphism, Single Nucleotide*

Grants and funding

G0601176/MRC_/Medical Research Council/United Kingdom