Real-time next-generation sequencing on shell-vial culture to contribute to diagnosis of lymphatic tuberculosis: a case report

Diagn Microbiol Infect Dis. 2021 Nov;101(3):115492. doi: 10.1016/j.diagmicrobio.2021.115492. Epub 2021 Jul 17.

Abstract

Lymph node tuberculosis is a of limited clinical suspicion form of Mycobacterium tuberculosis infection. After 15 days incubation in a cellular culture and directly from the supernatant, 11 minutes of Oxford Nanopore MinION sequencing provided a preliminary result of an antibiotic-susceptible M. tuberculosis Indo-Oceanic lineage strain. Oxford Nanopore MinION sequencing is a promising tool for optimising the laboratory diagnosis of lymph node tuberculosis.

Keywords: Lineage; Mycobacterium tuberculosis; Point-of-care; Real-time next-generation sequencing; Tuberculosis.

Publication types

  • Case Reports

MeSH terms

  • Clinical Laboratory Techniques / instrumentation
  • Clinical Laboratory Techniques / methods*
  • High-Throughput Nucleotide Sequencing / instrumentation
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Lymphatic Diseases / diagnostic imaging*
  • Lymphatic Diseases / microbiology*
  • Male
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / genetics*
  • Point-of-Care Testing
  • Tomography, X-Ray Computed
  • Tuberculosis / classification
  • Tuberculosis / diagnosis*
  • Tuberculosis / microbiology
  • Young Adult