Selection of extraction solvents for edible oils from microalgae and improvement of the oxidative stability

J Biosci Bioeng. 2021 Oct;132(4):365-371. doi: 10.1016/j.jbiosc.2021.06.008. Epub 2021 Jul 31.


Microalgae are natural, green raw material and could be used for the development of edible oil for its abundant polyunsaturated fatty acids, with fast growth rate. The wet mud and dry powder of Scenedesmus dimorphus were applied to compare the extraction effects of different organic solvent systems in this study. The results displayed that, by using the ethanol/n-hexane (3:2, v/v) mixed solvent, the oil extraction rate from wet algal mud was 68.31 %, with 71.65 % of neutral lipid, and 1.87 % of vitamin E; the retention rates of protein, chlorophyll, and carbohydrates in the algal residue after oil extraction were 60.56 %, 53.27 %, and 80.20 %, respectively. Through the single solvent n-hexane, the oil extraction rate from dried algal powder was 71.52 %, with 75.86 % of neutral lipids, and 1.63 % of vitamin E. The retention rates of protein, chlorophyll, and carbohydrate were 55.92 %, 61.33 % and 78.35 %, respectively, suggesting the high rate of nutrient retention. In addition, the orthogonal experiments indicated that the compound of low concentration natural antioxidants with 0.010 % of tea polyphenols, 0.005 % of vitamin E, and 0.015 % of rosemary extract had the best effects on improvement of oxidative stability.

Keywords: Edible oil; Extraction efficiency; Microalgal residue; Oxidative stability; Scenedesmus dimorphus.

MeSH terms

  • Biomass
  • Microalgae*
  • Oils
  • Oxidative Stress
  • Solvents


  • Oils
  • Solvents