Retrieval and purification of human β cells from stem cell-derived islet engrafted mice

STAR Protoc. 2021 Jul 15;2(3):100675. doi: 10.1016/j.xpro.2021.100675. eCollection 2021 Sep 17.

Abstract

Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a sufficient number of live β cells from mice following long-term human SC-β-cell engraftment. The protocol enables examination of SC-β-cells undergoing developmental and metabolic changes in vivo and may facilitate the understanding of metabolic demand on SC-β-cells.

Keywords: Cell Biology; Cell isolation; Organoids; Stem Cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Separation / methods*
  • Heterografts
  • Humans
  • Insulin-Secreting Cells / cytology*
  • Insulin-Secreting Cells / transplantation*
  • Islets of Langerhans Transplantation / methods
  • Mice
  • Stem Cells / cytology