The Saggy Ink Cap Medicinal Mushroom, Coprinus comatus (Agaricomycetes), Protein Attenuates Acute Alcoholic Liver Injury in Association with Changes in the Gut Microbiota of Mice

Int J Med Mushrooms. 2021;23(5):91-100. doi: 10.1615/IntJMedMushrooms.2021038229.


This study aims to investigate the hepatoprotective effects of Coprinus comatus protein (CCP) in a mouse model of acute alcoholic liver injury by regulating gut microbiota dysbiosis. Mice were divided into four groups, including the control group (CG), alcohol group (AG), biphenyldicarboxylate group (BG), and protein group (PG). The results showed that alcohol can increase the liver organ index, which could be adjusted by CCP. At the same time, analysis of serum biochemical indexes (alanine aminotransferase, aspartate aminotransferase) and liver oxidative stress levels (glutathione) revealed that CCP significantly alleviated alcohol-induced hepatic inflammation. Sequencing of 16S rRNA showed that gut microbiota composition was changed significantly by alcohol treatment. However, CCP could mitigate dysbiosis of gut microbiota, such as increasing the proportion of Muribaculaceae, Lachnospiraceae, and Lactobacillaceae and reducing the proportion of Burkholderiaceae, Deferribacteraceae, Enterococcaceae, and Enterobacteriaceae. In conclusion, CCP can maintain gut microbiota stability to improve liver injury and is potentially a good candidate for dietary supplements against acute alcoholic liver injury.

MeSH terms

  • Animals
  • Coprinus*
  • Gastrointestinal Microbiome*
  • Ink
  • Liver
  • Liver Diseases, Alcoholic* / drug therapy
  • Liver Diseases, Alcoholic* / prevention & control
  • Mice
  • RNA, Ribosomal, 16S / genetics


  • RNA, Ribosomal, 16S

Supplementary concepts

  • Coprinus comatus