Deacetylation of BmAda3 is required for cell apoptosis caused by Bombyx mori nucleopolyhedrovirus infection

Arch Insect Biochem Physiol. 2021 Oct;108(2):e21838. doi: 10.1002/arch.21838. Epub 2021 Aug 4.


Silkworm is not only an ideal insect model with a biological significance, but it is also crucially important in sericulture and bioreactors. Bombyx mori nucleopolyhedrovirus (BmNPV) is a principal pathogen of silkworm. However, the molecular mechanism underlying BmNPV invasion is still unclear. Based on our previous acetylome research findings of B. mori after BmNPV infection, here, we focused on silkworm alteration/deficiency in activation-3 (BmAda3). The acetylation of K124 and K128 were significantly reduced (0.66-fold) upon the virus challenge. Due to the interaction between Ada3 and P53, acetylation-mimic K124Q/K128Q and deacetylation-mimic K124R/K128R mutants of BmAda3 were constructed to explore the roles exerted by the acetylation modification of BmAda3 on P53. Yeast two-hybrid and IP results revealed that both BmAda3 and its deacetylation mutants (K124R/K128R) interacted with P53. Interestingly, we found that the deacetylation mutants (K124R/K128R) of BmAda3 significantly promoted the stability of P53. Since P53 is a proapoptotic factor, cell apoptosis was detected. We established that the deacetylation of BmAda3 at K124/K128 facilitated cellular apoptosis during BmNPV infection. Finally, viral proliferation was analyzed, and the results indicated that virus generation was reduced by K124/K128 deacetylation. Our report, based on the deacetylation of two lysine sites 124/128 of BmAda3, shows possible regulatory pathways of BmNPV proliferation and provides novel insights into the development of antiviral agents.

Keywords: BmAda3; BmNPV; P53 stability; apoptosis; deacetylation.

MeSH terms

  • Animals
  • Apoptosis*
  • Bombyx / genetics
  • Bombyx / metabolism
  • Bombyx / virology*
  • Genes, Insect
  • Histone Acetyltransferases* / genetics
  • Histone Acetyltransferases* / metabolism
  • Host-Pathogen Interactions
  • Insect Proteins / genetics
  • Insect Proteins / metabolism
  • Mutation
  • Nucleopolyhedroviruses / pathogenicity*
  • Tumor Suppressor Protein p53 / metabolism*


  • Insect Proteins
  • Tumor Suppressor Protein p53
  • Histone Acetyltransferases

Supplementary concepts

  • Bombyx mori nucleopolyhedrovirus