Cloning and characterization of tetM gene from a Ureaplasma urealyticum strain

J T Brown; M C Roberts

doi:10.1128/AAC.31.11.1852

Cloning and characterization of tetM gene from a Ureaplasma urealyticum strain

Antimicrob Agents Chemother. 1987 Nov;31(11):1852-4. doi: 10.1128/AAC.31.11.1852.

Authors

J T Brown¹, M C Roberts

Affiliation

¹ Department of Pathobiology, University of Washington, Seattle 98177.

Abstract

We have cloned a 4.9-kilobase (kb) HincII fragment which contains a tetracycline resistance determinant (tetM) from the chromosome of Ureaplasma urealyticum. The 4.9-kb HincII fragment contains DNA in addition to the structural gene, is closely related to the previously characterized 5.0-kb fragment from pJI3, and contains no normal ureaplasmal DNA sequences.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Cloning, Molecular*
DNA, Bacterial / analysis
Genes, Bacterial*
Nucleic Acid Hybridization
Tetracycline Resistance / genetics*
Ureaplasma / drug effects
Ureaplasma / genetics*

Substances

DNA, Bacterial

Abstract

Publication types

MeSH terms

Substances

Grants and funding