Detection of anticentromere antibodies using cloned autoantigen CENP-B

Arthritis Rheum. 1987 Dec;30(12):1416-9. doi: 10.1002/art.1780301214.

Abstract

A solid-phase enzyme-linked immunosorbent assay has been established using a cloned fusion protein, CtermCENP-B [beta-gal], as antigen. The fusion protein carries the major epitope of CENP-B, the major centromeric autoantigen. The enzyme-linked immunosorbent assay was more sensitive than immunofluorescence techniques in detecting anticentromere antibodies in patients with scleroderma or Raynaud's disease, and was weakly positive in 3% of normal controls and in 3% of 70 patients with other connective tissue diseases.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Autoantibodies / analysis*
  • Autoantigens / immunology*
  • Centromere / immunology*
  • Centromere Protein A
  • Chromosomal Proteins, Non-Histone*
  • Chromosomes / immunology*
  • Cloning, Molecular
  • Connective Tissue Diseases / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Male
  • Middle Aged
  • Nucleoproteins / immunology*

Substances

  • Autoantibodies
  • Autoantigens
  • Centromere Protein A
  • Chromosomal Proteins, Non-Histone
  • Nucleoproteins