Putting the pieces together: mapping the O-glycoproteome

Curr Opin Biotechnol. 2021 Oct;71:130-136. doi: 10.1016/j.copbio.2021.07.006. Epub 2021 Aug 4.


Protein glycosylation is the most diverse and omnipresent protein modification. Glycosylation provides glycoproteins with important structural and functional properties to facilitate critical biological processes. Despite the significance of protein glycosylation, the investigation of glycoproteome, especially O-linked glycoproteome, remains elusive due to the lack of a comprehensive methodology to conform with the diversity of O-linked glycoforms of O-linked glycoproteins. In recent years, mass spectrometry has become an indispensable tool for the characterization of O-linked glycosylated proteins across biological systems. We herein highlight the recent developments in MS-based O-linked glycoproteomic technologies, quantitative data acquisition strategy and bioinformatic tools, with a special focus on mucin-type O-linked glycosylation.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Glycoproteins* / metabolism
  • Glycosylation
  • Protein Processing, Post-Translational
  • Proteome* / metabolism


  • Glycoproteins
  • Proteome