Cyanidin-3-glucoside suppresses the progression of lung adenocarcinoma by downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway

World J Surg Oncol. 2021 Aug 6;19(1):232. doi: 10.1186/s12957-021-02339-7.

Abstract

Background: The aim of this study is to unravel the role of Cyanidin-3-glucoside (C3G) and its potential mechanisms in lung adenocarcinoma (LUAD).

Methods: The cell clones, proliferation, apoptosis, migration, and invasion in H1299 and A549 cells were determined by colony formation assay, 5-ethynyl-20 deoxyuridine (EdU) assay, flow cytometry, and transwell assay, respectively. The expression of p53-induced gene 3 (TP53I3) was assessed and the prognostic values of TP53I3 in LUAD via the dataset from the Cancer Genome Atlas (TCGA). In addition, the mRNA and protein expressions were detected by quantitative real-time PCR (qRT-PCR) and western blot.

Results: C3G inhibited the proliferation, migration, and invasion of, and also promoted the apoptosis in H1299 and A549 cells. The database of TCGA showed TP53I3 was highly expressed in LUAD tissues and correlated with the poor prognosis of LUAD patients. Moreover, we also found that C3G inhibited the proliferation, migration and invasion, and promoted apoptosis in H1299 and A549 cells by downregulating TP53I3. Additionally, C3G could inhibit the activation of phosphatidylinositol 3'-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway in H1299 and A549 cells by downregulating TP53I3.

Conclusion: This study demonstrated that C3G could inhibit the proliferation, migration and invasion, and also facilitate the apoptosis through downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway in LUAD.

Keywords: Apoptosis; C3G; LUAD; Metastasis; PI3K/AKT/mTOR pathway; TP53I3.

MeSH terms

  • Adenocarcinoma of Lung*
  • Anthocyanins
  • Apoptosis
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Lung Neoplasms* / drug therapy
  • Lung Neoplasms* / genetics
  • Phosphatidylinositol 3-Kinase / metabolism
  • Phosphatidylinositol 3-Kinases / metabolism
  • Prognosis
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-akt / metabolism
  • Signal Transduction
  • TOR Serine-Threonine Kinases / genetics
  • TOR Serine-Threonine Kinases / metabolism

Substances

  • Anthocyanins
  • Intracellular Signaling Peptides and Proteins
  • Phosphatidylinositol 3-Kinase
  • Phosphatidylinositol 3-Kinases
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-akt
  • TOR Serine-Threonine Kinases
  • TP53I3 protein, human
  • cyanidin-3-O-beta-glucopyranoside
  • MTOR protein, human