Dynamin deficiency causes insulin secretion failure and hyperglycemia

Proc Natl Acad Sci U S A. 2021 Aug 10;118(32):e2021764118. doi: 10.1073/pnas.2021764118.

Abstract

Pancreatic β cells operate with a high rate of membrane recycling for insulin secretion, yet endocytosis in these cells is not fully understood. We investigate this process in mature mouse β cells by genetically deleting dynamin GTPase, the membrane fission machinery essential for clathrin-mediated endocytosis. Unexpectedly, the mice lacking all three dynamin genes (DNM1, DNM2, DNM3) in their β cells are viable, and their β cells still contain numerous insulin granules. Endocytosis in these β cells is severely impaired, resulting in abnormal endocytic intermediates on the plasma membrane. Although insulin granules are abundant, their release upon glucose stimulation is blunted in both the first and second phases, leading to hyperglycemia and glucose intolerance in mice. Dynamin triple deletion impairs insulin granule exocytosis and decreases intracellular Ca2+ responses and granule docking. The docking defect is correlated with reduced expression of Munc13-1 and RIM1 and reorganization of cortical F-actin in β cells. Collectively, these findings uncover the role of dynamin in dense-core vesicle endocytosis and secretory capacity. Insulin secretion deficiency in the absence of dynamin-mediated endocytosis highlights the risk of impaired membrane trafficking in endocrine failure and diabetes pathogenesis.

Keywords: biphasic insulin secretion; diabetes; exocytosis–endocytosis coupling; readily releasable pool; vesicle recruitment.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / genetics
  • Blood Glucose / metabolism
  • Calcium Signaling / genetics
  • Dense Core Vesicles / metabolism
  • Dynamin II / genetics
  • Dynamins / genetics*
  • Dynamins / metabolism
  • Endocytosis / physiology
  • Female
  • GTP-Binding Proteins / metabolism
  • Hyperglycemia / etiology*
  • Insulin Secretion / physiology*
  • Insulin-Secreting Cells / metabolism*
  • Insulin-Secreting Cells / pathology
  • Male
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Nerve Tissue Proteins / metabolism

Substances

  • Blood Glucose
  • Nerve Tissue Proteins
  • Rims1 protein, mouse
  • Unc13a protein, mouse
  • GTP-Binding Proteins
  • DNM2 protein, mouse
  • Dynamin II
  • Dynamins