Distinct microbial communities colonize tonsillar squamous cell carcinoma

Oncoimmunology. 2021 Jul 25;10(1):1945202. doi: 10.1080/2162402X.2021.1945202. eCollection 2021.

Abstract

Squamous cell carcinoma of the tonsil is one of the most frequent cancers of the oropharynx. The escalating rate of tonsil cancer during the last decades is associated with the increase of high risk-human papilloma virus (HR-HPV) infections. While the microbiome in oropharyngeal malignant diseases has been characterized to some extent, the microbial colonization of HR-HPV-associated tonsil cancer remains largely unknown. Using 16S rRNA gene amplicon sequencing, we have characterized the microbiome of human palatine tonsil crypts in patients suffering from HR-HPV-associated tonsil cancer in comparison to a control cohort of adult sleep apnea patients. We found an increased abundance of the phyla Firmicutes and Actinobacteria in tumor patients, whereas the abundance of Spirochetes and Synergistetes was significantly higher in the control cohort. Furthermore, the accumulation of several genera such as Veillonella, Streptococcus and Prevotella_7 in tonsillar crypts was associated with tonsil cancer. In contrast, Fusobacterium, Prevotella and Treponema_2 were enriched in sleep apnea patients. Machine learning-based bacterial species analysis indicated that a particular bacterial composition in tonsillar crypts is tumor-predictive. Species-specific PCR-based validation in extended patient cohorts confirmed that differential abundance of Filifactor alocis and Prevotella melaninogenica is a distinct trait of tonsil cancer. This study shows that tonsil cancer patients harbor a characteristic microbiome in the crypt environment that differs from the microbiome of sleep apnea patients on all phylogenetic levels. Moreover, our analysis indicates that profiling of microbial communities in distinct tonsillar niches provides microbiome-based avenues for the diagnosis of tonsil cancer.

Keywords: 16S rRNA gene amplicon sequencing; Tonsillar squamous cell carcinoma; high risk-human papilloma virus (hr-hpv); microbiome; tonsil cancer; tonsillar microbiome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Squamous Cell*
  • Clostridiales
  • Humans
  • Microbiota* / genetics
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Tonsillar Neoplasms*

Substances

  • RNA, Ribosomal, 16S

Supplementary concepts

  • Filifactor alocis

Grant support

This study received financial support from the Swiss National Science Foundation (grant 182583), the Research Commission of the Kantonsspital St.Gallen (grant 16/22) and the San Salvatore Foundation, Lugano to B.L. The funders had no role in the study design, data collection, analysis, decision to publish, or preparation of the manuscript.