The effect of cisplatin-low-level laser therapy on cell viability and death of LNCaP prostate cancer cell line

Lasers Med Sci. 2022 Mar;37(2):1283-1288. doi: 10.1007/s10103-021-03386-y. Epub 2021 Aug 10.


Prostate cancer, as a common male cancer, is a serious threat to men's health. In spite of extreme developments for increasing survival rate, there are still limitations about common treatment options such as surgical procedures, radiotherapy, and chemotherapy. We hypothesized that combination of two treatments would bring better clinical outcomes. Therefore, the aim of this study was to determine the effect of conjugated cisplatin and low-level laser treatment (LLLT) on the viability of LNCaP prostate cancer cell line. LNCaP cells were harvested in DMEM containing 10% FBS and 1% antibiotic. Confluent cells were treated with different concentrations of cisplatin and different wavelengths of low-level laser (LLL) alone and in combination. The relative IC50 and cell viability was evaluated using MTT assay. Analysis of lipid peroxidation rate was performed using lipid peroxidation assay kit. LDH activity was also carried out on the treated and control cells using LDH cytotoxicity assay kit. Our results showed that combination of cisplatin and LLLT could effectively decrease cisplatin-induced cytotoxicity as well as LNCaP cell viability. Cisplatin-LLLT combination led to a significant increase in the MDA content as the product of membrane lipid peroxidation. Analyzing the LDH activity under the effect of cisplatin-LLL combined treatment showed a remarkable increase in the enzyme activity. We conclude that applying the cisplatin-LLL combination therapy is promising as an effective anti-cancer treatment. This novel combination has a potential to attenuate adverse side effects of earlier monotherapy strategies.

Keywords: Cisplatin; LNCaP cell line; Lactate dehydrogenase; Lipid peroxidation; Low-level laser therapy; Malondialdehyde.

MeSH terms

  • Antineoplastic Agents* / pharmacology
  • Antineoplastic Agents* / therapeutic use
  • Apoptosis
  • Cell Line, Tumor
  • Cell Survival
  • Cisplatin / pharmacology
  • Cisplatin / therapeutic use
  • Humans
  • Low-Level Light Therapy* / methods
  • Male
  • Prostatic Neoplasms* / drug therapy
  • Prostatic Neoplasms* / radiotherapy


  • Antineoplastic Agents
  • Cisplatin