Neuroprotective effects of 2-heptyl-3-hydroxy-4-quinolone in HT22 mouse hippocampal neuronal cells

Bioorg Med Chem Lett. 2021 Oct 1:49:128312. doi: 10.1016/j.bmcl.2021.128312. Epub 2021 Aug 8.

Abstract

The neuroprotective activity of 2-heptyl-3-hydroxy-4(1H)-quinolone (compound 1) was evaluated using the neurotoxicity of glutamate in the HT22 cell line. Compound 1, known as a signal molecule of the bacterial quorum-sensing system, protects neuronal cells from glutamate-induced neurotoxicity by inhibiting cellular Ca2+ uptake and glutamate-triggered ROS accumulation. MAPK signaling pathway inhibition by compound 1 was evaluated by immunoblotting the phosphorylation status of the proteins. Furthermore, pro-apoptotic protein levels and AIF translocation to the nucleus were found to be reduced by compound 1. In conclusion, compound 1 showed neuroprotective effects by inhibiting apoptotic neuronal cell death.

Keywords: Glutamate; HT22 hippocampal neuronal cells; MAPK; Neuroprotective effects; Quinolone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis Inducing Factor / metabolism
  • Cell Line
  • Cell Survival / drug effects
  • Glutamic Acid / pharmacology
  • MAP Kinase Signaling System / drug effects
  • Mice
  • Neuroprotective Agents / pharmacology*
  • Oxidative Stress / drug effects
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Quinolones / pharmacology*
  • Reactive Oxygen Species / metabolism
  • bcl-2-Associated X Protein / metabolism

Substances

  • 2-heptyl-3-hydroxy-4-quinolone
  • Apoptosis Inducing Factor
  • Bax protein, mouse
  • Neuroprotective Agents
  • AIFM1 protein, mouse
  • Proto-Oncogene Proteins c-bcl-2
  • Quinolones
  • Reactive Oxygen Species
  • bcl-2-Associated X Protein
  • Bcl2 protein, mouse
  • Glutamic Acid